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Discover Sandwich Elisa Protocol at Antibody Elisa

Enzyme Linked Immunosorbent Assay (ELISA) is a powerful technique for detection and quantitation of biological substances such as protein, peptides, antibodies, and hormones. By combining the specificity of antibodies with the sensitivity of simple enzyme assay, ELISA can provide a quick and useful measurement of the concentration of an unknown antigen or antibody. The Boster Bio protocol for ELISA offers a step-by-step overview of the procedure. At you will find all the information related to Elisa.

Currently, there are three major types of ELISA assays commonly used by researchers. They are: indirect ELISA, typically used for screening antibodies; sandwich ELISA protocol (or antigen capture), for analysis of antigen present; and competitive ELISA, for antigen specificity. PBL's IFN ELISA kits use the sandwich strategy.

The "sandwich" technique is so called because the antigen being assayed is held between two different antibodies each recognizing particular epitopes on the antigen. Because of both antibodies are specific for the target protein, this method can increase the assay specificity two to five times over other direct or indirect detection methods. In this method:

Elisa Troubleshooting is easy at ELISA is the gold standard of in vitro diagnostics (IVD) during the last five decades for analysis of biomarkers and important analyses in healthcare and diversified analytical settings. With over 300,000 peer-reviewed articles to date, ELISA-based technologies have open up a lucrative, commercial market. Despite ongoing developments in immunosensors, labs-on-chips, and microfluidic and point-of-care technologies, ELISA with high throughput and omnipotent nature has been unmatched in reliability for the monitoring and management of disease markers. It is still the most widely used immunoassay format by pharmaceutical industries for routine monitoring of drugs and drug impurities.


Modern medicine recognizes the complications of treating infectious diseases. It is realized that early diagnosis of diseases is extremely important these days.

Connecting particular proteins to specific disease continues to pose challenges to science even today, though a lot of developments have taken place in past. ELISA can be used to probe numerous diseases in several organisms. The ELISA is a simple test that produces dependable data in a short span of time. ELISA or Enzyme- link immunosorbent assay is the technique used to detect the presence of antibodies in the blood. In some instances, this test is not just for patience but also for plant pathology and quality control in various industries.

by: Antibody Elisa

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